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A single-molecule understanding of transcriptional bursting

Date 16 May 2022 Time 11:00 - 12:00
Location AMOLF Lecture Room and Virtual
Speaker Tineke Lenstra (Netherlands Cancer Institute (AvL))
Category Public Colloquium

Transcriptional in single cells is a stochastic process, which arises from the random collision of molecules. This stochastic behaviour results in variability in gene activity between cells, and as well as within a cell over time. In our lab, we use single-molecule imaging techniques to visualize the dynamics of transcription in living cells. We and others have shown that genes are often transcribed in bursts, with periods of high activity interspersed by periods of inactivity. Transcriptional bursting is observed from bacteria to yeast to human cells, but the origin and regulators of bursting are still largely unknown. In this talk, I will focus on how transcriptional bursting in yeast is regulated by transcriptional activators, DNA supercoiling and nucleosomes.